Elastin polypeptide-coated small-diameter vascular grafts. by Adrienne Michele Lovink

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In this work, we examined the feasibility of using recombinant human elastin polypeptide (EP) to coat e-PTFE vascular grafts. First, EP was cross-linked using: glutaraldehyde, lysine diisocyanate, and pyrolloquinoline quinone. Second, we attempted to cross-link EP coatings applied to e-PTFE vascular grafts. Acceptable coatings were achieved through passive adsorption from 70% ethanol and DMSO solutions. Unfortunately, coating cross-linking with glutaraldehyde or pyrolloquinoline quinone resulted in an inappropriate topography for the desired application. The use of gamma irradiation resulted in an ideal cross-linked EP coating. However, gamma irradiation is not recommended with e-PTFE. Finally, we preliminarily assessed the smooth muscle cell (SMC) and endothelial cell (EC) response to our EPs. [3H] thymidine uptake experiments showed slower SMC proliferation on EP compared to growth on gelatin. In addition, our analysis shows lower collagen and elastase secretions indicating that the presence of EP does not appear to upregulate the cells in culture.

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Pagination170 leaves.
Number of Pages170
ID Numbers
Open LibraryOL19512318M
ISBN 100612952150

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Small-diameter vascular grafts (SDVGs) with inner lumen diameter (d) less than 6 mm are required in vascular reconstructive surgery. Tissue engineering (TE) represents an emerging research field where the production of vascular grafts. Elastin-like Polypeptide Enriched Surfaces for Cardiovascular Applications Currently used small diameter synthetic vascular grafts are prone to high rates of failure related to thrombosis and Author: Patrick Herve Blit.

The Lack of Organized Elastin in Vascular Grafts. Elastin polypeptide-coated small-diameter vascular grafts. book The annual mortality associated with cardiovascular disease worldwide is expected to increase from million in to million in Author: Ziyu Wang, Linyang Liu, Suzanne M.

Mithieux, Anthony S. Weiss. Progress in understanding elastogenesis, the process by which elastin is naturally synthesized, and increased access to synthetic elastin-based materials, has increased Elastin polypeptide-coated small-diameter vascular grafts.

book usage of elastin in vascular graft engineering. In this chapter, we explore recent advances in the utilization of elastin as a material for vascular graft Author: Richard Wang, Bente J. De Kort, Anthal I. Smits, Anthony S. Weiss. The media comprises of e.g. collagene and elastin, with a dense population of smooth muscle cells (SMCs) that are oriented perpendicular to the blood flow.

The adventitia, the outer layer of the artery, Cited by: 1. (ID = mm) diameter vascular grafts [6]. However, small diameter (ID grafts of the same materials fail years post implantation [5][7]due to luminal narrowing caused by thrombosis [8] and.

vascular grafts is in patients with renal failure who depend on dialysis. Autologous arteries and veins, as well as synthetic grafts, are currently used as grafts for replacing small diameter blood vessels. However, some of these grafts have a limited life time.

For example, saphenous veins, used as coronary bypass grafts. Patent small diameter vascular grafts in vivo Vascular conduits ( mm i.d.) were implanted in a rat aortic interposition model for 1–14 days (Fig.

4 A and B). The choice of – g. Elastin-mimetic peptide polymers have been synthesized, and the morphological properties of fabricated small diameter fibers and nonwoven fabrics have been characterized.

An 81 kDa recombinant protein based upon the repeating elastomeric peptide sequence of elastin. materials of prosthetic vascular grafts. Examined by means of decades of use, both Dacron and ePTFE grafts have been shown to perform well at diameters 6 mm, but neither material has been suitable for small-diameter (4 mm) applications.

Thus, finding a solution for small-diameter. Electrospinning of the PDO and elastin-blended composite into a conduit for use as a small diameter vascular graft has extreme potential and warrants further investigation as it thus far compares favorably to native vessel.

Tissue engineered vascular grafts (TEVGs) have the potential to overcome the issues faced by existing small diameter prosthetic grafts by providing a biodegradable scaffold where the.

SMCs and collagen/elastin deposition in electrospun vascular grafts at 1-month post-implantation. (A)–(C) Cross-sectional staining of SM-MHC for SMCs in the vascular grafts. (D)–(F) Collagen and elastin deposited in the grafts were examined by using Verhoeff's stain.

Collagen is shown in pink, Elastin. A series of small-diameter vascular grafts consisting of an elastin-like protein reinforced with controlled volume fractions and orientations of synthetic collagen fiber was designed and prototyped.

Gong W, Lei D, Li S, et al. Hybrid small-diameter vascular grafts: anti-expansion effect of electrospun poly epsilon-caprolactone on heparin-coated decellularized matrices. In this study, hybrid small-diameter vascular grafts made of thermoplastic polyurethane (TPU) and silk fibroin, which possessed a hybrid fibrous structure of an aligned inner layer and a random outer layer.

Advances in cardiovascular materials have brought us improved artificial vessels with larger diameters for reducing adverse responses that drive acute thrombosis and the associated complications. Nonetheless, the challenge is still considerable when applying these materials in small-diameter blood vessels.

Here we report the biomimetic design of an acellular small-diameter vascular graft. The gold standard vascular substitutes, used in cardiovascular surgery, are the Dacron or expanded polytetrafluoroethylene (ePTFE)-derived grafts.

However, major adverse reactions accompany their use. For this purpose, decellularized human umbilical arteries (hUAs) may be proven as a significant source for the development of small diameter. Thus far, construction of small-diameter vascular substitutes with both endothelial and muscular tissues, which is essential for the grafts to acquire antithrombosis function and sufficient.

The intima, the inner layer of the artery that is in contact with blood, consists of a monolayer of endothelial cells supported by an underlying connective tissue membrane.

The media comprises of e.g. collagene and elastin. Summary. In order to evaluate tissue and cell infiltration and endothelialization behaviors of small diameter polyurethane (PU) vascular prosthesis, PU grafts ( mm ID, 1 cm in length) prepared by.

Introduction. Synthetic grafts are widely used in cardiovascular surgery as conduits for arterial bypass in large- and medium-sized vessels. Unfortunately, use of synthetic grafts in small diameter applications (small diameter. Abstract (summary): Currently used small diameter synthetic vascular grafts are prone to high rates of failure related to thrombosis and neointimal hyperplasia.

Biomimetic materials, based. Vascular smooth muscle cells (VSMCs) play important roles in the physiology and pathophysiology of the blood vessels.

In a healthy adult organism, VSMCs are quiescent, but after a blood vessel injury, they. This updatable book aims to cover all the relevant aspects of Vascular Tissue Engineering (VTE) and novel alternatives to develop vascular grafts for clinical applications.

The chapters in this book cover. Enriched elastin vascular grafts generated by decellularization and removal of collagen with proteases from porcine carotid arteries can support fibroblasts in vitro (Chuang, Stabler et al.

Cell infiltration has also been observed for other decellularized vascular. Biotechnology approaches to repair and replace arteries have been under development for more than a century. Early synthetic approaches used rubber-based replacements, which then evolved into the.

Since there are still limitations in the replacement of small diameter vascular grafts, the need and demand for developing more desirable grafts is increasing day by day. In this study, polycaprolactone small-diameter (6 mm) vascular grafts. Tissue-engineered vascular grafts require elastic, acellular porous scaffolds with controlled biodegradability and properties matching those of natural arteries.

Elastin would be a desirable component for such applications, but elastin. Although collagen-based tissue-engineered blood vessels (TEBVs) have many interesting properties and have been utilized to study aspects of vascular biology, these constructs are too weak to be implanted as bypass grafts for in vivo investigations.

This study presents a method to incorporate organized, intact elastin. Small diameter (4mm), thin-walled, non-ringed ePTFE vascular grafts (W. Gore, Flagstaff, AZ) were cut to 7 cm in length and coated with a thin layer of silicone glue on the last cm of each end.

Graft. One of the main open issues in modern vascular surgery is the nonbiodegradability of implants used for stent interventions, which can lead to small caliber-related thrombosis and neointimal hyperplasia.

Using a technique known as electrospinning, researchers were able to manipulate the PDO-elastin composite into a conduit, or hose, for use as a small diameter vascular graft. "We have created a. The vascular biomaterials can be fabricated from synthetic or natural polymers for vascular tissue engineering.

Stem cells can be seeded by different techniques into tissue-engineered vascular grafts in vitro and implanted in vivo to repair the vascular.

Preparation of fibrin-based TEV constructs. SMC containing thrombin was mixed with fibrinogen at volume ratio resulting in a final cell concentration of × 10 6 cells/ml. The final. "Small-diameter vascular grafts, or grafts involving blood vessels with an internal diameter smaller than five millimeters, have much higher failure rates than larger ones," says Dr.

Vande Geest. The leading cause of failure in vascular grafts has been attributed to thrombosis, or the formation of blood clots. Seeding small-diameter vascular graft constructs with cells genetically engineered to secrete anti-thrombotic factors may improve graft.

1. Introduction. Vascular disease is the major cause of death in Western society. In the USA alone, more than arterial bypass operations using autogenous grafts are conducted per.

Elastin-like polypeptides (ELPs) have found utility in tissue engineering applications, not only because they are biocompatible, biodegradable, and non-immunogenic, but also because their amino acid. Electrospinning Collagen and Elastin: Preliminary Vascular Tissue Engineering,” Front Biosci., 9 (2), pp.

Development of Biomimetic Thermoplastic Polyurethane/Fibroin Small-Diameter Vascular Grafts. The Potential to Create Small Diameter Bioresorbable Vascular Grafts Through Electrospinning.

Journal of Materials Chemistry, Sell, S.A. and G.L. Bowlin. A Novel Suture-Reinforced Electrospun Polydioxanone-Elastin Small-Diameter Tube for Use in Vascular .Tissue engineering of small diameter (vascular grafts.

It involves in vitro seeding of cells onto a scaffold .Elastin is the molecule responsible for the elasticity of blood vessel walls. With age, progressive loss of elasticity of vessels occurs, presumably because of fragmentation of the elastin molecule.

Read More; lung. In human aging: Respiratory system. The lung also contains elastin .

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